TECHNOLOGY OF BOVINE LEUKEMIA VIRUS GENODIAGNOSTICS IN CATTLE, IN PRODUCED RAW MATERIALS AND PRODUCTS

The most important task of the dairy cattle industry is to obtain high quality raw milk. To achieve it, a set measures required, including aimed at increasing biological safety produced materials. aim study was create scientific and methodological basis for Bovine leukemia virus (BLV) gene diagnostics in combined format pathogen indication identification. This required updating strategy BLV PCR-RFLP genotyping, consistent with its phylogenetic classification, taking into account growing knowledge about genetic diversity 11 genotypes studied viral pathogen. When staging nested PCR, oligonucleotide primers were used, which initiate final stage reaction production 444 bp env-gene fragment Five restriction endonucleases used genotyping of: PvuII, SspI, AsuHPI, HaeIII, BstX2I. As result verification developed method identification an updated strategy, technical obtained, expressed ability identify all discovered date by interpreting generated 58 genotype-associated combinations profiles.